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Image Search Results
Journal: Matrix biology : journal of the International Society for Matrix Biology
Article Title: Molecular and epigenetic ex vivo profiling of testis cancer-associated fibroblasts and their interaction with germ cell tumor cells and macrophages.
doi: 10.1016/j.matbio.2024.06.001
Figure Lengend Snippet: Fig. 4. (A) Visualization of the RNAseq expression data and (B) LC-MS-based secretome data of IGFBP1 / IGFBP1, LGALS3BP / LGALS3BP, LYVE1 / LYVE1, and PTX3 / PTX3 in CAF populations compared to nFB, and (C) qRT-PCR-based validation of IGFBP1, LGALS3BP, LYVE1, and PTX3 expression in nFB and CAF (SE-CAF = 6, EC- CAF = 3, TER-CAF = 3, nFB = 5). SD is based on biological replicates. GAPDH and ACTB were used as housekeepers and for data normalization. (D) Measurement of LGALS3BP secretion in nFB and CAF via ELISA, dots indicate the biological replicates (n = 3 / subgroup). (E) Cell proliferation assay of TCam-2 (SE) and 2102EP, NCCIT, NT2/D1 (EC) treated daily with recombinant proteins (LGALS3BP, LYVE1, IGFBP1; 10 - 100 ng / mL) over 10 d. SE-related SD is based on technical triplicates; EC-related SD is based on biological triplicates (each in technical triplicates). (F) qRT-PCR analysis of expression of cisplatin resistance-associated factors in TCam-2 (SE) and 2102EP, NCCIT, NT2/D1 (EC) treated daily with recombinant proteins (LGALS3BP, LYVE1, IGFBP1; 10 - 100 ng / mL) over 10 d SE-related SD is based on technical triplicates; EC-related SD is based on biological triplicates (each in technical triplicates). GAPDH and ACTB were used as housekeepers and for data normalization. Dashed lines indicate the threshold of a FC > 1.5 / < -1.5.
Article Snippet: For TCam-2, 1300 cells and for EC cells, 2650 cells per well of a 24- well plate were seeded, treated daily with
Techniques: Expressing, Liquid Chromatography with Mass Spectroscopy, Quantitative RT-PCR, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Proliferation Assay, Recombinant
Journal: Matrix biology : journal of the International Society for Matrix Biology
Article Title: Molecular and epigenetic ex vivo profiling of testis cancer-associated fibroblasts and their interaction with germ cell tumor cells and macrophages.
doi: 10.1016/j.matbio.2024.06.001
Figure Lengend Snippet: Fig. 5. (A) Experimental setup of this analysis. THP-1 monocytes were differentiated into M0 macrophages by PMA. Afterwards, macrophages were cultivated in CM or treated with recombinant proteins, subsequently expression of M1LPS/IFNγ and M2IL4/IL13 marker genes was analyzed by qRT-PCR. (B) Heatmap of gene expression levels (FC to control) of THP-1-M0-macrophages cultivated in nFB, SE- / EC-CAF CM (n = 3 / each) or (C) treated with recombinant proteins (LGALS3BP, LYVE1, PTX3; 10 - 100 ng / mL) over 72 h. GAPDH and ACTB were used as housekeepers and for data normalization. Average overall expression intensities were used for clustering. Arrows highlight genes used for futher analysis.
Article Snippet: For TCam-2, 1300 cells and for EC cells, 2650 cells per well of a 24- well plate were seeded, treated daily with
Techniques: Recombinant, Expressing, Marker, Quantitative RT-PCR, Gene Expression, Control